Praziquantel Tablets (Finished product) Assay (By HPLC)
Mobile Phase - Acetonitrile : Water ( 45:55 )
Standard Preparation: Take 100 mg of Praziquantel WS in 100 ml of volumetric flask. Add 50 ml of mobile phase and sonicate to dissolve the contents. Dilute upto the mark with mobile phase. Take 10 ml of the above solution and dilute to 50 ml with mobile phase.
Sample Preparation: Weigh accurately about powder equivalent to 100 mg of Praziquantel in 100 ml of volumetric flask. Add 50 ml of mobile phase and sonicate to dissolve the contents completely then dilute upto the mark with mobile phase.
Take 10 ml of the above solution and
dilute to 50 ml with mobile phase.
Chromatographic Conditions :
Column Size - C18 ( 250 x 4.0 mm ) 5ยต or
equivalent
Detector Wavelength - 210 nm
Flow Rate - 1.0 ml/min.
Injection volume - 20 ml.
Run time - 25
min.
Retention time of praziquntel - About 13 minutes
Procedure : Inject one blank , five replicate injection of standard solution, two replicate injection of sample solution & two bracketing standard solution.
System Suitability:
1) RSD of
five replicate injection of standard solution should not be more than 2.0%
2) RSD
of five replicate injection of
standard solution and two
bracketing standard solution should not
be more than 5.0%
3) Tailing factor should not be more than 2.0
Calculation:
