Saturday, 4 September 2021

Phenazone Assay (By UV) Finished Product | Test Procedure | Method of analysis (MOA) |



Phenazone (Finished product) Assay (By UV)


Standard Preparation: Weigh accurately 125 mg of Phenazone WS in 100 ml volumetric flask add 50 ml distilled water, sonicate for two minutes and make up the volume with distilled water. Take 1 ml of above solution and dilute to 100 ml with distilled water.

Sample Preparation: Weigh accurately about sample equivalent to 125 mg of Phenazone WS in 100 ml volumetric flask add 50 ml distilled water, sonicate for two minutes and make up the volume with distilled water. Take 1 ml of above solution and dilute to 100 ml with distilled water.

Measure the absorbance Standard and Test solution at 242 nm

 

Calculation :