Meloxicam
Injection Assay (MOA) By HPLC
Sample Preparation
Weigh sample
equivalent 40 mg of Meloxicam in 100 ml of volumetric flask, add
0.3 ml of 0.4 M sodium hydroxide and shake well
and dilute to 100 ml with Methanol ( 400 mcg )
Standard Preparation
Take 40 mg of Meloxicam WS in 100 ml
volumetric flask and dilute with methanol upto 100 ml ( 400 mcg )
Chromatographic Conditions
Column - C18 (10
cm × 4.0 mm) 10 µm or equivalent
Flow Rate
- 1.0 ml/minute.
Column temperature - Ambient
Detection wavelengths - 350 nm.
Inject - 10 µL of
each solution.
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Time Mobile Phase A Mobile Phase B Comment
(
Minutes) (% v/v ) (% v/v )
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0 – 2.5 60 40 Isocratic
2.5 - 12 60 → 30 40 → 70 Linear Gradient
12 - 25 30 70 Isocratic
25 - 26 30 → 60 70 → 40 Linear Gradient
26 - 30 60 40 Re- equilibration
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Calculation
