Vitamin E (Finished product) Assay (By HPLC)
Standard Preparation: Take 10 mg of vitamin E WS in 100 ml volumetric flask, add diluent to dissolve, and make up volume upto the mark with diluent.
Sample Preparation: Weigh sample equivalent to 6.8 IU 100 ml volumetric flask, add 70 ml of diluents and sonicate for 30 minutes, and make up volume upto mark with diluent, shake well and filter.
Mobile phase: Methanol : Water ( 98 : 2 )
Chromatographic Conditions:
Column - C15 l = 15 cm, Ǿ = 3.9 mm
Detector wavelength - 254 nm
Flow rate - 1.0 ml / Minute
Injection - 200 μl
Diluent : Methanol
Procedure : Inject one blank , five replicate injection of Standard solution, two replicate injection of sample solution & two bracketing standard solution.
System Suitability:
1) RSD of five replicate injection of standard solution should not be more than 2.0%
2) RSD of five replicate injection of standard solution and two bracketing standard solution should not be more than 5.0%
3) Tailing factor should not be more than 2.0
Calculation :
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