Mobile Phase: Prepare a solution
of Acetonitrile, Water and Triethylamine in the ratio (40:60:0.1), adjust the
pH 2.5 with phosphoric acid, filter and degas.
Standard Preparation: Dissolve, accurately
about 100 mg of Ofloxacin WS & 250 mg Ornidazole with Mobile phase and
dilute to 100 ml flask. Take 2 ml of the diluted solution in a dry 50 ml flask and
dilute it to the mark with the Mobile phase.
Sample Preparation: Weigh and powdered
20 tablets. Take powder equivalent to 100 mg of Ofloxacin & 250 mg of
Ornidazole in 100 ml dry flask and add 50 – 60 ml of Mobile phase, sonicate for
10 mins. dilute upto mark with Mobile phase & shake. Take 2 ml of the
diluted solution in a dry 50 ml flask and dilute it to the mark with Mobile
phase.
Chromatographic
Column :
4.6 mm X 25 cm Zorbax Rx- C8 column
Wavelength
: 300 nm
Flow rate :
1 ml per minute
Procedure: Separately inject
about 20 µl of Standard and Sample preparation into the chromatograph and
record the chromatograms. Measure the area for the major peak.
Calculation :
Ofloxacin=
Ornidazole=
