Using Paddle, Use as
the medium 900 mL of phosphate buffer pH 5.8 and rotate the paddle at 50
revolutions per minute. Withdraw a sample of 10 mL of the medium, filter
and dilute the filtrate with sufficient of the dissolution medium to give a
solution expected to contain about 0.001% w/v of Furosemide. Measure the absorbance
of this solution, at 277 nm using dissolution medium in the
reference cell. Calculate the total content of furosemide, C12H11ClN2O5S,
in the medium from the absorbance obtained from a 0.001% w/v solution of furosemide
ws in the dissolution medium and using the declared content of C12H11ClN2O5S
in furosemide ws.
(1) Sample: withdraw a 40 mL sample of the
medium and filter. Dilute 25 mL of the filtrate to 100 mL with the phosphate
buffer pH 5.8, to produce a solution expected to contain 0.001% w/v of furosemide.
(2) Standard: 0.001% w/v furosemide ws
in phosphate buffer pH 5.8. Take 100 mg quantity of the furosemide ws with 70
mL of the phosphate buffer pH 5.8 for 10 minutes, dilute to 100 mL with the
same solvent, filter through a glass-fibre filter (Whatman GF/C is suitable)
and dilute 1 mL of the filtrate to 100 mL with the phosphate buffer pH 5.8.
Dissolution (%)
