Dexamethasone phosphate Injection Assay (MOA) By HPLC
Mobile phase: To 1.360 gm of potassium dihydrogen phosphate add 0.600 g of hexylamine, mix, allow to stand for 10 minutes, dissolve in 182.5 ml of water, add 67.5 ml of acetonitrile, mix and filter (0.45 µm).
Standard
Preparation
Weigh 131.60 mg of Dexamethasone phosphate WS equivalent to 100 mg of Dexamethasone in 100 ml volumetric flask, add 50 ml of mobile phase and sonicate to dissolve dexamethasone phosphate completely and dilute to 100ml with mobile phase. Shake well and further dilute 5 ml to 50 ml with mobile phase.
Sample Preparation
Weigh and transfer injection
equivalent to 10 mg of
Dexamethasone In 100 ml of volumetric flask, add 50 ml of mobile phase,
sonicate if required for 5 mins to dissolve dexamethasone phosphate completely
and dilute to 100ml with mobile phase. Shake well
Chromatographic conditions
Flow Rate - 1 ml per minute.
Column Temperature - Ambient
Detection
wavelength - 254 nm.
Inject - 20 µL of
each solution.
Determination
of Content
Calculate the content of C22H29FO5 in the injection from the peak areas obtained and from the declared content of C22H30FO8P in dexamethasone phosphate. 1 mg of C22H30FO8P is equivalent to 0.831 mg of C22H29FO5.
Calculation
