Reagents: 1
M Hydrochloric acid
(85 ml hydrochloric acid in 1000 ml in water.)
Sample
Dilution: Weigh and powder 20 tablets. Take powder equivalent to 100 mg of
theophyliine add 5 ml of 1 N hydrochloric acid and 25 ml of water, shake well
for 30 minutes and dilute to 100 ml with water. Take 1 ml of the solution in
100 ml of volumetric flask & dilute with water. (10 mcg/ml)
Take reading on suitable Spectrophotometer at 271 nm using water as a
blank.
Calculation:
