Reagents: (1)
1.0% w/v 4-Amino Antipyrine in Ethanol.
(2) 8.0%w/v solution of Potassium Ferricyanide in water.
(3) 4.0% w/v solution of Sodium Bicarbonate in water.
Spl Dilution : Weigh and powdered 20 Tablets Take powder equivalent to about 2 mg in 50 ml (water)
Procedure
: To 10ml each of sample and standard solutions, add 50 ml water & 4
ml of 4% Sodium Bicarbonate solution add 4 ml of 1.0% w/v 4-Amino Antipyrine in
Ethanol shake to mix, add 4 ml of 8% Potassium Ferricyanide solution. Allow to
stand of 15 min. and then extract with 20, 10, 10 ml portions of chloroform.
Pass chloroform layer through a layer of Anhydrous Sodium Sulphate and make up
to 50 ml with chloroform. Measure the extinction of each solution at about 463
nm using chloroform as blank.
Calculate the results by comparing with standard.
