Test solution
: Test (1) To a quantity of the powdered tablets containing 2.5 mg of
Dexamethasone add 20 mL of methanol (50%), shake & Sonicate for
20 minutes and filter through glass-fibre filter (Whatman GF/C is
suitable).
Standard
solution : (2) 0.0125% w/v of dexamethasone ws in methanol (50%). Weigh accurately
about 50 mg of Dexamethasone in 100 ml volumetric flask add 50 ml of
methanol (50%) shake & Sonicate for atleast 20 minutes & dilute
to 100 ml with methanol . Further dilute 5 ml to 20 ml with methanol (50 %)
CHROMATOGRAPHIC
CONDITIONS:
Use a stainless (a) steel column (20 cm × 4.6 mm) packed with
octadecylsilyl silica gel for chromatography (5 µm) (Spherisorb ODS 1 is
suitable).
(b) Use phase described
below. isocratic elution and the mobile
(c) Use a flow rate of 1.4 mL per minute.
(d) Use an ambient column temperature.
(e) Use a detection wavelength of 238 nm.
(f) Inject 20 µL of each solution.
DETERMINATION OF
CONTENT
Calculate the content of C22H29FO5 in the tablets using the declared
content of C22H29FO5 in dexamethasone ws.
