Enrofloxacin Injection Assay (MOA) By HPLC
Preparation of Buffer: 0.025M Phosphoric acid previously
adjusted pH 3.0 with Triethlyamine.
Mobile Phase - Buffer : Acetonitrile (87 : 13)
Standard Preparation
Take 50 mg of
Enrofloxacin WS in 100 ml volumetric flask, add 30 ml mobile phase, sonicate to
dissolve and make up volume upto mark with mobile phase
Sample Preparation
Weigh and transfer liquid sample equivalent to 50 mg of Enrofloxacin in 100 ml volumetric flask, add 30 ml of
mobile phase, sonicate for 10 minutes, and make up volume up to mark with
mobile phase and Shake.
Chromatographic Conditions :
Column - Size - C18( 250 x 4.6mm ) 5μm
Detector wavelength -
278 nm
Flow Rate - 1.5 ml / Minute
Injection volume - 20 μl.
Column Temp - 30°C
Procedure : Inject one blank , five replicate injection of standard solution, two replicate injection of sample solution & two bracketing standard solution.
System Suitability :
1) RSD of five replicate injection of standard solution should not be more than 2.0%
2) RSD of five replicate injection of standard solution and two bracketing standard solution should not be more than 2.0%
3) Tailing factor should not be more than 2.0
Calculation :
