Solution
preparation:
Mobile phase: 5 ml of trifluoroacetic
acid, 150ml of acetonitrile and 345 volumes of water.
Standard
solution: Weigh
accurately about 25 mg of Tramadol Hydrochloride working standard in 100 ml
volumetric flask, add 70 ml mobile phase, sonicate it 10 minutes , dilute to
the volume with mobile phase.
Test solution: Weigh and transfer __ mg of the powder,
equivalent to 25 mg of Tramadol Hydrochloride, to a 100-ml volumetric flask.
Add 70 ml of mobile phase, sonicate it half an hours, dilute with mobile phase
to volume, mix, filter.
CHROMATOGRAPHIC CONDITIONS:
Column : Use
a stainless steel column (25 cm × 4.6 mm) (5 µm) ( C18 ).
Flow rate : 1.0 ml/min.
Injection volume : 20 µl
Column temperature: Ambient
Wavelength : 271 nm.
Method
: Isocratic
Procedure: Inject the
blank (one time), standard solution (six times) and test solution (two times)
The relative standard deviation of six
replicate standards should not be more than 2 %.
Calculation
The content per capsule is calculated by the equation
Calculation :
