Solution preparation :
Mobile Phase: Mix 470 ml of methanol and
530 ml of water.
0.002% w/v of hydrocortisone
: Dissolve 20 mg of hydrocortisone in 100 ml
Methanol (50%), Dilute 25 ml of this solution to 250 ml with Methanol (50%).
Standard Solution : Accurately weigh and transfer
25 mg of betamethasone WS
and 20 mg of hydrocortisone...
